ABSTRACT
In this study, the effect of agitation and aeration rates on xylanase activity of Aspergillus niger SS7 in 3-litre stirred tank bioreactor was investigated. The agitation rates tested were 100, 200 and 300 rpm at each airflow rates of 0.5, 1.0 and 1.5 vvm. The maximum xylanase activity in mono- agitator system was at the agitation speed of 200 rpm and aeration rate of 1.0 vvm. In bi-agitator system, at low agitation speed (100 rpm), the xylanase activity was enhanced by 13 percent compared to mono- agitator system for an aeration rate of 1.0 vvm. Xylanase productivity in continuous culture was higher by approximately 3.5 times than in batch culture.
ABSTRACT
The xylanase production by Cochliobolus sativus strain Cs6 was improved under solid state fermentation (SSF). High xylanase activity (1079 U/g) was obtained when wheat straw was used after 8 days of incubation. Combinations of sodium nitrate with peptone or yeast extract resulted in an increased xylanase production (1543 and 1483 U/g, respectively). The Cs6 strain grown in SSF in a simple medium, proved to be a promising microorganism for xylanase production.
A produção de xilanase por Cochliobolus sativus cepa Cs6 SATIVUS por fermentação em estado sólido (SSF) foi melhorada. Com o emprego de palha de trigo, obteve-se elevada atividade de xilanase (1079 U/g) após 8 dias de incubação. Combinações de nitrato de sódio com peptona ou extrato de levedura aumentaram a produção de xilanase (1543 e 1483 U/g, respectivamente). Comprovou-se que a cepa Cs6, cultivada em SSF em meio simples, é um microrganismo promissor para produção de xilanase.